籍贯

长沙

出生年月

1989.10

学历

博士

职称

副教授

Email

yangzhan@hunau.edu.cn

电话

15874174276

招生

意向

招生类型

招生学科

研究方向

硕  士

临床兽医学

兽医临床微生物学及功能蛋白质的研究

兽医硕士

兽医学

兽医临床分子诊断及流行病学调查

教育

经历

2012.09-2017.07，湖南农业大学，临床兽医学博士（硕博连读）   

2014.09-2015.02，美国国立卫生研究院，访问学者

2008.09-2012.07，湖南农业大学，动物医学学士

研究

项目

1.     自科类横向项目，2022430118001428，猪重要病毒性疾病疫苗及检测试剂盒开发，2022/09-2024/12，200万，在研，主持

2.     湖南省青年科技人才项目，2022RC1046，2022年度湖南省“三尖”创新人才工程项目，2022/10认定

3.     国家自然科学基金项目，32202790，PCV2衣壳表面IDR区域正电荷氨基酸残基对病毒吸附与复制的作用研究，2023/01-2025/12，30万，在研，主持

4.     湖南省自然科学基金项目，2022JJ40183，PCV2衣壳蛋白质插入PPV抗原表位的展示策略及免疫原性研究，2022/01-2024/12，5万，在研，主持

5.     长沙市自然科学基金项目，kq2202231，Loop GH和羧基端嵌合PPV抗原表位对PCV2免疫原性的影响研究，2022/01-2023/12，5万，在研，主持

6.     湖南省教育厅科学研究项目，18B092，猪圆环病毒2 型核衣壳五倍轴区域附近氨基酸残基的生物学功能研究，2019/01-2020/12，5万，已结题，主持

7.     湖南省技术攻关“揭榜挂帅”项目，2021NK1030，非洲猪瘟等重要疫病蛋白质颗粒疫苗研发，2022/01-2024/12，2500万，在研，参与（第三）

发表

论文

1.            Yuan Chen^#, Shile Luo^#,   Jianmei Tan, Luhua Zhang, Shengwu Qiu, Zhiyou Hao, Naidong Wang, Zhibang   Deng, Aibing Wang, Qing Yang, Yi Yang, Changjian Wang^*, Yang   Zhan^*. Establishment and application of multiplex real-time   PCR for simultaneous detection of four viruses associated with porcine   reproductive failure. Front Microbiol. 2023, 14. (published   online)

2.            Yang Zhan^*, Luhua   Zhang, Yuan Lin, Yunfeng Cai, Yawen Zou, Zhiyou Hao, Zhanhua Luo, Naidong Wang,   Zhibang Deng, Yi Yang, Changjian Wang, Qing Yang^*. Development and   preliminary testing of a probe-based duplex real-time PCR assay for the   detection of African swine fever virus. Mol Cell Probes. 2021, 59:   101764.

3.            Yang   Zhan^#, Wanting Yu^#, Xiong   Cai, Xinnuo Lei, Hongyu Lei, Aibing Wang, Yujie Sun, Naidong Wang, Zhibang   Deng, Yi Yang^*. The carboxyl terminus (CT) of the PCV2 capsid protein (Cap) is   critical to VLP assembly, cell entry, and propagation. J Virol. 2020, 94(9): e00042-20.

4.            Yang   Zhan,   Naidong Wang, Zhe Zhu, Zhanfeng Wang, Aibing Wang, Zhibang Deng, Yi Yang^*.   In silico analyses of antigenicity and surface structure variation of an   emerging PCV2b mutant, prevalent in southern China from 2013 to 2015. J Gen Virol. 2016, 97(4): 922-933.  

5.            Haoyang Yu^#, Luhua Zhang^#,   Yunfeng Cai, Zhiyou Hao, Zhanhua Luo, Tao Peng, Lei Liu, Naidong Wang,   Guiping Wang, Zhibang Deng, Yang Zhan^*. Seroprevalence of   antibodies to classical swine fever virus and porcine reproductive and   respiratory syndrome virus in healthy pigs in Hunan Province, China. Pol   J Vet Sci. 2022, 25(3): 375-381.

6.            Naidong Wang^#, Yang Zhan^#, Aibing Wang,   Lijie Zhang, Reza Khayat, Yi Yang^*. In silico Analysis of Surface   Structure Variation of PCV2 Capsid Resulted from Loops Mutation of Its Capsid   Protein (Cap). J Gen Virol. 2016, 97(12): 3331-3344.（SCI, IF=3.192）

7.            Yi Hu^#, Yang Zhan^#, Dongliang   Wang, Xiaohong Xie, Tanbin Liu, Wei Liu, Naidong Wang, Zhibang Deng, Hongyu   Lei, Yi Yang, Aibing Wang^*. Evidence of natural co-infection of   PCV2b subtypes in vivo. Arch Virol. 2017, 162(7):   2015-2020.（SCI,   IF=2.058） 

8.            Wanting Yu, Yang Zhan, Boxin Xue, Yanpeng Dong, Yanfeng Wang, Ping Jiang,   Aibing Wang, Yujie Sun^*, Yi Yang^*. Highly efficient   cellular uptake of a cell-penetrating peptide (CPP) derived from the capsid   protein of porcine circovirus type 2. J Biol Chem. 2018, 293(39): 15221-15232.

9.            Yi Hu, Xiong Cai, Yang Zhan, Xiaomin Yuan, Tanbin Liu, Lei Tan, Yalan Li, Lijie   Zhang, Lingchen Yang, Wei Liu, Zhibang Deng, Naidong Wang, Yi Yang, Shiyin Guo^*   and Aibing Wang^*. Truncated Rep protein of porcine circovirus 2   (PCV2) caused by a naturally occurring mutation reduced virus replication in   PK15 cells. BMC Vet Res. 2019, 15(1): 248.

10.         Yan Zhang, Zhanfeng Wang, Yang Zhan, Qian Gong, Wanting Yu,   Zhibang Deng, Aibing Wang, Yi Yang, Naidong Wang^*. Generation of   E. coli-derived virus-like particles of porcine circovirus type 2 and their   use for an indirect IgG Enzyme-Linked Immunosorbent Assay. Arch   Virol. 2016, 161(6): 1485-1491.

11.         Qian Gong^#, Yi Hu^#,   Yang Zhan, Dongliang Wang, Naidong   Wang, Guanyu Chen, Aibing Wang, Yi Yang^*. Complete Genome Sequence   of Porcine Circovirus Strain YiY-3-2-H5 with a novel insertion, Isolated from   Hunan Province, China. Genome Announc. 2016, 4(2).

12.         Zhe Zhu^#, Naidong Wang^#,   Yang Zhan, Zhanfeng Wang, Aibing   Wang, Zhibang Deng^*, Yi Yang^*. Complete genome   sequence of porcine circovirus strain 102 with a novel mutation, isolated   from hunan province, china. Genome Announc.   2015, 3(3).

13.         Qing Li^#, Qiaoling Yuan^#,   Tianjie Wang, Yang Zhan, Lingchen Yang, Ying Fan, Hongyu Lei^*,   Jianming Su^*. Fumonisin B1 Inhibits Cell Proliferation and   Decreases Barrier Function of Swine Umbilical Vein Endothelial Cells. Toxins.   2021, 13(12): 863.

14.         Jie Cai, Xiaohong Xie, Yi Hu, Yang Zhan, Wanting Yu, Aibing Wang, Naidong   Wang^*. Generation of PCV2 in PK15 cells transfected with recombinant   baculovirus containing a 1.1 copy of the PCV2 genome. Acta Veterinaria Hungarica.   2017, 65(2): 278-290.

15.         Lei Lei, Fan Liao, Lei Tan, Deyong   Duan, Yang Zhan, Naidong Wang, Yuge Wang, Xiaoye Peng, Kaixin Wang, Xiaojiu   Huang, Yi Yang^*, Aibing Wang^*. LAMP Coupled   CRISPR-Cas12a Module for Rapid, Sensitive and Visual Detection of Porcine   Circovirus 2. Animals. 2022, 12(18): 2413.

16.         Dongliang Wang, Jinhui Mai, Wenfeng   Zhou, Wanting Yu, Yang Zhan, Naidong   Wang, Neal D. Epstein^*, Yi Yang^*. Immunoinformatic Analysis   of T- and B-Cell Epitopes for SARS-CoV-2 Vaccine Design. Vaccines. 2020, 8(3):   355.

17.         Lingchen Yang, Di Tu, Naidong Wang,   Zhibang Deng, Yang Zhan, Wei Liu,   Yi Hu, Tanbin Liu, Lei Tan, Yalan Li, Shiyin Guo^*, Aibing Wang^*.   The protective effects of DL-Selenomethionine against T-2/HT-2 toxinsinduced   cytotoxicity and oxidative stress in broiler hepatocytes. Toxicol   In Vitro. 2019, 54: 137-146.

18.         Jiaxin Zhang, Xinnuo Lei, Dongliang Wang,   Yifan Jiang, Yang Zhan, Meng Li, Yawen   Zhou, Yiwen Qin, Jue Liu, Aibing Wang, Yi Yang, Naidong Wang^*.   Inhibition of Abl or Src tyrosine kinase decreased porcine circovirus type 2   production in PK15 cells. Res Vet Sci. 2019. 124: 1-9.

19.         Tanbin Liu^#, Yi Hu^#,   Shiyin Guo, Lei Tan, Yang Zhan, Lingchen   Yang, Wei Liu, Naidong Wang, Yalan Li, Yingfan Zhang, Chengyu Liu, Yi Yang,   Robert S. Adelstein, Aibing Wang^*. Identification and characterization   of MYH9 locus for high efficient gene knock-in and stable expression in mouse   embryonic stem cells. PLoS One. 2018, 13(2): e0192641.

20.         Naidong Wang, Yan Zhang, Xinnuo Lei,   Wanting Yu, Yang Zhan, Dongliang   Wang, Jiaxin Zhang, Aibing Wang, Lehui Xiao, Ping Jiang^*, Yi Yang^*.   Optimized conditions for preserving stability and integrity of porcine   circovirus type 2 virus-like particles during long-term storage. Journal   of Virological Methods. 2017, 243: 146-150.

21.         Dongliang Wang, Sujiao Zhang, Yawen Zou,   Wanting Yu, Yifan Jiang, Yang Zhan, Naidong Wang^*, Yanpeng   Dong, Yi Yang^*. Structure-Based Design of Porcine Circovirus Type   2 Chimeric VLPs (cVLPs) Displays Foreign Peptides on the Capsid Surface. Front   Cell Infect Microbiol. 2018, 8: 232.

22.         Wanting Yu, Yuao Sun, Qing He,   Chaoying Sun, Tian Dong, Luhua Zhang, Yang Zhan, Naidong Wang, Yi Yang,   Yujie Sun^*. Mitochondrial Localization Signal of Porcine Circovirus   Type 2 Capsid Protein Plays a Critical Role in Cap-Induced Apoptosis. Vet   Sci. 2021, 8(11): 272.

23.         Naidong Wang, Sujiao Zhang, Dongliang   Wang, Fuqiang Li, Lin Liang, Xiuli Li, Yawen Zou, Yang Zhan, Guanyu Chen, Wanting Yu, Zhibang Deng, Di Tu^*,   Shangjin Cui^*. Protective humoral immunity in guinea pigs induced   by PCV2 virus-like particles displaying the B cell linear epitope (²²⁸QQITDA²³³)   of PPV1. Vet Microbiol. 2019, 235: 86-92.

24.         Lingchen Yang, Di Tu, Yingxin Wu, Wei   Liu, Yi Hu, Tanbin Liu, Lei Tan, Yalan Li, Hongyu Lei, Yang Zhan, Naidong Wang, Zhibang Deng, Shiyin Guo^*, Aibing Wang^*. Distribution and   persistence of residual T-2 and HT-2 toxins from moldy feed in broiler   chickens. Toxicon. 2020, 178: 82-91.

25.         Lei Tan, Yalan Li, Jiayi He, Yi Hu,   Xiong Cai, Wei Liu, Tanbing Liu, Jiaoshun Wang, Zhoumian Li, Xiaoming Yuan, Yang Zhan, Lingchen Yang, Zhibang   Deng, Naidong Wang, Yi Yang, Aibing Wang^*. Epidemic and genetic   characterization of porcine epidemic diarrhea virus strains circulating in   the regions around Hunan, China, during 2017-2018. Arch Virol. 2020, 165(4):   877-889.

26.         张露华，李周勉，唐祎依，蔡云凤，田唯嘉，黄坤，王东亮，王乃东，湛洋^*. 猪圆环病毒2型和3型双重PCR方法的建立与优化. 黑龙江畜牧兽医. 2020，(05): 71-76.

27.         贺延峰，王东亮，邹亚文，董彦鹏，王乃东，湛洋^*. 湖南地区猪圆环病毒2型的分离与鉴定. 中国兽医科学. 2019, 49(02):   213-219.

28.         黄坤，王东亮，邹亚文，王乃东，董彦鹏，湛洋^*. 猪圆环病毒2d 型的分子生物学特征和流行病学研究进展. 经济动物学报. 2019, 23(01):   51-56.

29.         陈冠宇，蔡云凤，张露华，罗占华，王乃东，湛洋^*. 猪圆环病毒2型编码蛋白调控细胞凋亡机制研究进展. 畜牧兽医科学(电子版). 2021(17): 1-4.

30.         湛洋，王东亮，王乃东，蒋一凡，黄坤，崔尚金，姜平，杨毅^*. 猪圆环病毒3型检测及其Cap结构序列和抗原性预测分析. 畜牧兽医学报. 2017, 48(6): 1076-1084.

31.         湛洋，胡意，王东亮，蔡杰，邓治邦^*.   PCV2a-ORF3基因突变株感染性克隆的构建. 中国预防兽医学报. 2017, 39(5): 351-355.

32.         王东亮，湛洋，胡意，雷昕诺，庞培，张素娇，王爱兵，杨毅，王乃东^*. 猪圆环病毒2型衣壳蛋白的Loops结构及病毒样颗粒制备的研究进展.中国兽医学报. 2018(1): 235-240.

33.         余婉婷, 湛洋, 雷昕诺, 张颜, 谢小红, 王乃东, 邓治邦, 王爱兵, 杨毅^*. 猪圆环病毒2型病毒样颗粒的研究及应用. 中国畜牧兽医文摘. 2015, 31(5): 57-58.

34.         陈指龙，邓吉平，湛洋，林 源，王昌建，谭艳芳，杨青^*，袁安文^*.   猪圆环病毒2型湖南株的分离鉴定与进化分析. 中国兽医科学. 2020, 50(12):   1522-1530.

35.         李亚兰, 袁晓民, 湛洋, 杨凌宸, 胡意, 谭磊, 刘谭彬, 郭时印, 王爱兵^*. 猪流行性腹泻病毒检测方法的研究进展. 中国兽医科学. 2019, 49(03):   347-354.

36.         谷婷，庞培，湛洋，奉湘艳，徐奇，刘沅，贺延峰，方满新，邓治邦^*.   PCV2病毒载量与病理损伤的相关性. 中国兽医学报. 2018, 38(04):   625-630+649.

37.         王占锋, 朱哲, 湛洋, 邓治邦, 王爱兵, 杨毅, 王乃东^*. 猪圆环病毒2型湖南分离株HNLYYA1全基因组的多引物滚环扩增和序列分析. 中国兽医科学. 2016(4): 454-460.

38.         廖帆，谭磊，雷磊，湛洋，段德勇，王爱兵. 猪圆环病毒2型LAMP可视化检测方法的建立与应用. 中国兽医科学. 2022, 52(06):   679-684.

39.         王教顺, 王东亮, 张素姣, 江游, 湛洋, 袁晓民, 邓治邦^*. PCV2b与PCV2d病毒样颗粒交叉免疫保护效力的评估[C].   中国畜牧兽医学会兽医病理学分会, 2019:22.

40.         张佳鑫, 蒋一凡, 雷昕诺, 秦艺文, 湛洋, 王乃东^*. Src和Abl酪氨酸蛋白激酶家族参与病原微生物感染的研究进展. 微生物学通报. 2019, 46(10):   2781-2786.

41.         张素姣, 王东亮, 李萌, 蒋一凡, 湛洋, 王乃东^*. Loop   EF区嵌合猪细小病毒B细胞表位对猪圆环病毒2型病毒样颗粒组装的影响. 中国兽医科学. 2019, 49(02):   176-182.

42.         李周勉, 邹亚文, 蒋一凡, 胡意, 秦艺文, 李萌, 湛洋, 王东亮, 王乃东^*. 猪圆环病毒3型Cap蛋白序列的生物信息学研究. 经济动物学报. 2020, 24(01):   9-16.

获奖

成果

2021.07  湖南农业大学第七届“互联网+”大学生创新创业大赛青红旅赛道创意组二等奖（本人为指导教师）

2020.04  2019年度湖南省自然科学奖二等奖（第二完成人）（猪圆环病毒2型衣壳蛋白loop结构及其展示外源抗原表位的研究）

2016.12  “大北农杯”第一届全国农林院校研究生学术科技作品一等奖（新型PCV2b突变毒株表面结构的模拟及PCV2重组进化分析）

2016.06  美国微生物学大会优秀博士生奖学金（In silico analyses of   antigenicity and surface structure variation of an emerging PCV2b mutant）

社会

兼职

第九届中国畜牧兽医学会兽医内科与临床诊疗学分会理事 (201908-202307)